We use applied statistics, machine learning and efficient algorithms to address fundamental problems in biology and medicine. We develop computational methods for better design and integrative analysis of high-throughput functional genomic assays and perturbation experiments, with a focus on multi-modal single-cell technologies. We apply these methods to study regulatory genomics of cell function and cell-cell interactions *in vivo* in close collaboration with experimental biologists, with a focus on immunology and cancer.

Selected publications

Yuri Pritykin*, Joris van der Veeken*, Allison Pine, Yi Zhong, Merve Sahin, Linas Mazutis, Dana Pe’er, Alexander Rudensky, Christina Leslie (2021). A unified atlas of CD8 T cell dysfunctional states in cancer and infection. Molecular Cell. https://doi.org/10.1016/j.molcel.2021.03.045

Xiaoyi Li*, Yuri Pritykin*, Carla P Concepcion*, Yuheng Lu, Gaspare La Rocca, Minsi Zhang, Bryan King, Peter J Cook, Yu Wah Au, Olesja Popow, Joao A.Paulo, Hannah G Otis, Chiara Mastroleo, Paul Ogrodowski, Ryan Schreiner, Kevin M Haigis, Doron Betel, Christina S Leslie, Andrea Ventura (2020). High-Resolution in vivo identification of miRNA targets by Halo-enhanced Ago2 pull-down. Molecular Cell. https://doi.org/10.1016/j.molcel.2020.05.009

Chrysothemis C Brown, Herman Gudjonson, Yuri Pritykin, Deeksha Deep, Vincent-Philippe Lavallée, Alejandra Mendoza, Rachel Fromme, Linas Mazutis, Charlotte Ariyan, Christina Leslie, Dana Pe’er, Alexander Y Rudensky (2019). Transcriptional basis of mouse and human dendritic cell heterogeneity. Cell. https://doi.org/10.1016/j.cell.2019.09.035

Catherine Konopacki*, Yuri Pritykin*, Yury Rubtsov, Christina S. Leslie, Alexander Y. Rudensky (2019). Transcription factor Foxp1 regulates Foxp3 chromatin binding and coordinates regulatory T cell function. Nature Immunology. https://doi.org/10.1038/s41590-018-0291-z

Alexendar R Perez*, Yuri Pritykin*, Joana A Vidigal*, Sagar Chhangawala, Lee Zamparo, Christina S Leslie, Andrea Ventura (2017). GuideScan software for improved single and paired CRISPR guide RNA design. Nature Biotechnology. https://doi.org/10.1038/nbt.3804

* equal contribution