TitleUncoupling neurogenic gene networks in the Drosophila embryo.
Publication TypeJournal Article
Year of Publication2017
AuthorsRogers, WA, Goyal, Y, Yamaya, K, Shvartsman, SY, Levine, MS
JournalGenes Dev
Volume31
Issue7
Pagination634-638
Date Published2017 Apr 01
ISSN1549-5477
KeywordsAnimals, Cell Lineage, Cells, Cultured, Central Nervous System, CRISPR-Cas Systems, Drosophila, Drosophila Proteins, Embryo, Nonmammalian, Epidermal Growth Factor, Female, Gene Expression Regulation, Developmental, Gene Regulatory Networks, Male, Membrane Proteins, Neurogenesis, Receptor, Epidermal Growth Factor, Receptors, Invertebrate Peptide, Signal Transduction
Abstract

The EGF signaling pathway specifies neuronal identities in the Drosophila embryo by regulating developmental patterning genes such as intermediate neuroblasts defective (ind). EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand, which is processed by the Rhomboid protease. CRISPR/Cas9 was used to delete defined rhomboid enhancers mediating expression at each site of Spitz processing. Surprisingly, the neurogenic ectoderm, not the ventral midline, was found to be the dominant source of EGF patterning activity. We suggest that Drosophila is undergoing an evolutionary transition in central nervous system (CNS)-organizing activity from the ventral midline to the neurogenic ectoderm.

DOI10.1101/gad.297150.117
Alternate JournalGenes Dev.
PubMed ID28428262
PubMed Central IDPMC5411704
Grant ListR01 GM086537 / GM / NIGMS NIH HHS / United States
R35 GM118147 / GM / NIGMS NIH HHS / United States