TitleTranscriptional interpretation of the EGF receptor signaling gradient.
Publication TypeJournal Article
Year of Publication2012
AuthorsFuchs, A, Cheung, LS, Charbonnier, E, Shvartsman, SY, Pyrowolakis, G
JournalProc Natl Acad Sci U S A
Volume109
Issue5
Pagination1572-7
Date Published2012 Jan 31
KeywordsAnimals, Base Sequence, DNA, Drosophila, Gene Expression Regulation, Oogenesis, Receptor, Epidermal Growth Factor, Signal Transduction, Transcription, Genetic
Abstract

Epidermal growth factor receptor (EGFR) controls a wide range of developmental events, from body axes specification in insects to cardiac development in humans. During Drosophila oogenesis, a gradient of EGFR activation patterns the follicular epithelium. Multiple transcriptional targets of EGFR in this tissue have been identified, but their regulatory elements are essentially unknown. We report the regulatory elements of broad (br) and pipe (pip), two important targets of EGFR signaling in Drosophila oogenesis. br is expressed in a complex pattern that prefigures the formation of respiratory eggshell appendages. We found that this pattern is generated by dynamic activities of two regulatory elements, which display different responses to Pointed, Capicua, and Mirror, transcription factors involved in the EGFR-mediated gene expression. One of these elements is active in a pattern similar to pip, a gene repressed by EGFR and essential for establishing the dorsoventral polarity of the embryo. We demonstrate that this similarity of expression depends on a common sequence motif that binds Mirror in vitro and is essential for transcriptional repression in vivo.

Alternate JournalProc. Natl. Acad. Sci. U.S.A.