|Title||The temporal requirement for endothelin receptor-B signalling during neural crest development.|
|Publication Type||Journal Article|
|Year of Publication||1999|
|Authors||Shin, MK, Levorse, JM, Ingram, RS, Tilghman, SM|
|Date Published||1999 Dec 2|
|Keywords||Animals, Cecum, Cell Differentiation, Cell Movement, Colon, Doxycycline, Gene Expression Regulation, Developmental, Melanocytes, Mice, Mice, Inbred C57BL, Neural Crest, Neurons, Receptor, Endothelin B, Receptors, Endothelin, Signal Transduction, Tetracycline, Trans-Activators|
Endothelin receptor B (EDNRB) is a G-protein-coupled receptor with seven transmembrane domains which is required for the development of melanocytes and enteric neurons. Mice that are homozygous for a null mutation in the Ednrb gene are almost completely white and die as juveniles from megacolon. To determine when EDNRB signalling is required during embryogenesis, we have exploited the tetracycline-inducible system to generate strains of mice in which the endogenous Ednrb locus is under the control of the tetracycline-dependant transactivators tTa or rtTA. By using this system to express Ednrb at different stages of embryogenesis, we have determined that EDNRB is required during a restricted period of neural crest development between embryonic days 10 and 12.5. Moreover, our results imply that EDNRB is required for the migration of both melanoblasts and enteric neuroblasts.