The pre-vertebrate origins of neurogenic placodes. Author Philip Abitua, Blair Gainous, Angela Kaczmarczyk, Christopher Winchell, Clare Hudson, Kaori Kamata, Masashi Nakagawa, Motoyuki Tsuda, Takehiro Kusakabe, Michael Levine Publication Year 2015 Type Journal Article Abstract The sudden appearance of the neural crest and neurogenic placodes in early branching vertebrates has puzzled biologists for over a century. These embryonic tissues contribute to the development of the cranium and associated sensory organs, which were crucial for the evolution of the vertebrate "new head". A previous study suggests that rudimentary neural crest cells existed in ancestral chordates. However, the evolutionary origins of neurogenic placodes have remained obscure owing to a paucity of embryonic data from tunicates, the closest living relatives to those early vertebrates. Here we show that the tunicate Ciona intestinalis exhibits a proto-placodal ectoderm (PPE) that requires inhibition of bone morphogenetic protein (BMP) and expresses the key regulatory determinant Six1/2 and its co-factor Eya, a developmental process conserved across vertebrates. The Ciona PPE is shown to produce ciliated neurons that express genes for gonadotropin-releasing hormone (GnRH), a G-protein-coupled receptor for relaxin-3 (RXFP3) and a functional cyclic nucleotide-gated channel (CNGA), which suggests dual chemosensory and neurosecretory activities. These observations provide evidence that Ciona has a neurogenic proto-placode, which forms neurons that appear to be related to those derived from the olfactory placode and hypothalamic neurons of vertebrates. We discuss the possibility that the PPE-derived GnRH neurons of Ciona resemble an ancestral cell type, a progenitor to the complex neuronal circuit that integrates sensory information and neuroendocrine functions in vertebrates. Keywords Animals, Molecular Sequence Data, Humans, Ciona intestinalis, Larva, Receptors, G-Protein-Coupled, Body Patterning, Neurons, Ectoderm, Homeodomain Proteins, Bone Morphogenetic Proteins, Intracellular Signaling Peptides and Proteins, HEK293 Cells, Protein Tyrosine Phosphatases, Vertebrates, Gonadotropin-Releasing Hormone Journal Nature Volume 524 Issue 7566 Pages 462-5 Date Published 08/2015 Alternate Journal Nature Google ScholarBibTeXEndNote X3 XML