Phylogenetic portrait of the Saccharomyces cerevisiae functional genome. Author Patrick Gibney, Mark Hickman, Patrick Bradley, John Matese, David Botstein Publication Year 2013 Type Journal Article Abstract The genome of budding yeast (Saccharomyces cerevisiae) contains approximately 5800 protein-encoding genes, the majority of which are associated with some known biological function. Yet the extent of amino acid sequence conservation of these genes over all phyla has only been partially examined. Here we provide a more comprehensive overview and visualization of the conservation of yeast genes and a means for browsing and exploring the data in detail, down to the individual yeast gene, at http://yeast-phylogroups.princeton.edu. We used data from the OrthoMCL database, which has defined orthologs from approximately 150 completely sequenced genomes, including diverse representatives of the archeal, bacterial, and eukaryotic domains. By clustering genes based on similar patterns of conservation, we organized and visualized all the protein-encoding genes in yeast as a single heat map. Most genes fall into one of eight major clusters, called "phylogroups." Gene ontology analysis of the phylogroups revealed that they were associated with specific, distinct trends in gene function, generalizations likely to be of interest to a wide range of biologists. Keywords Animals, Saccharomyces cerevisiae, Cluster Analysis, Databases, Genetic, Saccharomyces cerevisiae Proteins, Genome, Fungal, Phylogeny Journal G3 (Bethesda) Volume 3 Issue 8 Pages 1335-40 Date Published 08/2013 Alternate Journal G3 (Bethesda) Google ScholarBibTeXEndNote X3 XML