Optimizing the detection of nascent transcripts by RNA fluorescence in situ hybridization. Author C van Raamsdonk, S Tilghman Publication Year 2001 Type Journal Article Abstract An unusual feature of the mammalian genome is the number of genes exhibiting monoallelic expression. Recently random monoallelic expression of autosomal genes has been reported for olfactory and Ly-49 NK receptor genes, as well as for Il-2, Il-4 and Pax5. RNA fluorescence in situ hybridization (FISH) has been exploited to monitor allelic expression by visualizing the number of sites of transcription in individual nuclei. However, the sensitivity of this technique is difficult to determine for a given gene. We show that by combining DNA and RNA FISH it is possible to control for the hybridization efficiency and the accessibility and visibility of fluorescent probes within the nucleus. Keywords Animals, Cells, Cultured, Gene Expression Regulation, Developmental, In Situ Hybridization, Fluorescence, Mice, Transcription, Genetic, RNA, Messenger, DNA, Cell Nucleus, Repressor Proteins, Homeodomain Proteins, Fluorescent Dyes, Sensitivity and Specificity, Alleles, Eye Proteins, Paired Box Transcription Factors, Retinal Ganglion Cells Journal Nucleic Acids Res Volume 29 Issue 8 Pages E42-2 Date Published 04/2001 Alternate Journal Nucleic Acids Res. Google ScholarBibTeXEndNote X3 XML
