Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate. Author Sanford Silverman, Allegra Petti, Nikolai Slavov, Lance Parsons, Ryan Briehof, Stephan Thiberge, Daniel Zenklusen, Saumil Gandhi, Daniel Larson, Robert Singer, David Botstein Publication Year 2010 Type Journal Article Abstract Oscillations in patterns of expression of a large fraction of yeast genes are associated with the "metabolic cycle," usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that these oscillations happen in single cells drawn from unsynchronized cultures growing exponentially in chemostats. Gene-expression data from synchronized cultures were used to predict coincident appearance of mRNAs from pairs of genes in the unsynchronized cells. Quantitative analysis of the FISH results shows that individual unsynchronized cells growing slowly because of glucose limitation or phosphate limitation show the predicted oscillations. We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source. Keywords In Situ Hybridization, Fluorescence, Glucose, Gene Expression Profiling, Gene Expression Regulation, Fungal, Saccharomyces cerevisiae, Models, Biological, Phosphates, RNA, Messenger, Energy Metabolism, Genes, Fungal, Oscillometry, Cell Division Journal Proc Natl Acad Sci U S A Volume 107 Issue 15 Pages 6946-51 Date Published 04/2010 Alternate Journal Proc. Natl. Acad. Sci. U.S.A. Google ScholarBibTeXEndNote X3 XML