Isolation of sea squirt (Ciona) gametes, fertilization, dechorionation, and development. Author Lionel Christiaen, Eileen Wagner, Weiyang Shi, Michael Levine Publication Year 2009 Type Journal Article Keywords Animals, Embryo, Nonmammalian, Embryonic Development, Female, Male, Zygote, In Situ Hybridization, Ciona intestinalis, Models, Animal, Ovum, Spermatozoa, Chorion, Embryo Culture Techniques, Fertilization, Life Cycle Stages Journal Cold Spring Harb Protoc Volume 2009 Issue 12 Pages pdb.prot5344 Date Published 12/2009 Alternate Journal Cold Spring Harb Protoc Google ScholarBibTeXEndNote X3 XML