Identification of alterations in DNA copy number in host stromal cells during tumor progression. Author Robert Pelham, Linda Rodgers, Ira Hall, Robert Lucito, Ken Nguyen, Nicholas Navin, James Hicks, David Mu, Scott Powers, Michael Wigler, David Botstein Publication Year 2006 Type Journal Article Abstract The interactions between cancer cells and the surrounding host stromal tissue play a critical role in tumor progression and metastasis, but the molecular nature of this relationship remains largely uncharacterized. Furthermore, although genetic changes of neoplastic cells in tumors contribute significantly to tumor progression, it is not known whether similar changes occur in the adjacent host stromal microenvironment and whether they contribute to or inhibit tumorigenesis. To address this question in an unbiased and genome-wide manner, we applied high-resolution DNA copy number analysis to murine stromal DNA isolated from human xenograft tumors that were formed in immunodeficient mice. We show that numerous amplifications and deletions are found within the host stromal microenvironment, suggesting that alterations in host DNA copy number can occur and may play a significant role in modifying tumor-stromal interactions. Keywords Animals, Mice, Humans, Genome, DNA, Gene Dosage, Neoplasms, Cell Line, Tumor, Disease Progression, Mice, Nude, Neoplasm Transplantation, Stromal Cells Journal Proc Natl Acad Sci U S A Volume 103 Issue 52 Pages 19848-53 Date Published 12/2006 Alternate Journal Proc. Natl. Acad. Sci. U.S.A. Google ScholarBibTeXEndNote X3 XML