Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts. Author J Rossi, D Burke, J Leung, D Koos, H Chen, S Tilghman Publication Year 1992 Type Journal Article Abstract A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17. Keywords Animals, Female, Base Sequence, Mice, Molecular Sequence Data, Genetic Markers, Saccharomyces cerevisiae, Genome, DNA, Chromosome Mapping, Polymerase Chain Reaction, Mice, Inbred C57BL, Chromosomes, Fungal, Gene Library, Exons, Oligodeoxyribonucleotides, Polymorphism, Restriction Fragment Length, Spleen Journal Proc Natl Acad Sci U S A Volume 89 Issue 6 Pages 2456-60 Date Published 03/1992 Alternate Journal Proc. Natl. Acad. Sci. U.S.A. Google ScholarBibTeXEndNote X3 XML
