Gene expression patterns in ovarian carcinomas. Author Marci Schaner, Douglas Ross, Giuseppe Ciaravino, Therese Sorlie, Olga Troyanskaya, Maximilian Diehn, Yan Wang, George Duran, Thomas Sikic, Sandra Caldeira, Hanne Skomedal, I-Ping Tu, Tina Hernandez-Boussard, Steven Johnson, Peter O'Dwyer, Michael Fero, Gunnar Kristensen, Anne-Lise Borresen-Dale, Trevor Hastie, Robert Tibshirani, Matt van de Rijn, Nelson Teng, Teri Longacre, David Botstein, Patrick Brown, Branimir Sikic Publication Year 2003 Type Journal Article Abstract We used DNA microarrays to characterize the global gene expression patterns in surface epithelial cancers of the ovary. We identified groups of genes that distinguished the clear cell subtype from other ovarian carcinomas, grade I and II from grade III serous papillary carcinomas, and ovarian from breast carcinomas. Six clear cell carcinomas were distinguished from 36 other ovarian carcinomas (predominantly serous papillary) based on their gene expression patterns. The differences may yield insights into the worse prognosis and therapeutic resistance associated with clear cell carcinomas. A comparison of the gene expression patterns in the ovarian cancers to published data of gene expression in breast cancers revealed a large number of differentially expressed genes. We identified a group of 62 genes that correctly classified all 125 breast and ovarian cancer specimens. Among the best discriminators more highly expressed in the ovarian carcinomas were PAX8 (paired box gene 8), mesothelin, and ephrin-B1 (EFNB1). Although estrogen receptor was expressed in both the ovarian and breast cancers, genes that are coregulated with the estrogen receptor in breast cancers, including GATA-3, LIV-1, and X-box binding protein 1, did not show a similar pattern of coexpression in the ovarian cancers. Keywords Female, Humans, Immunohistochemistry, Trans-Activators, Transcription Factors, Oligonucleotide Array Sequence Analysis, DNA-Binding Proteins, Breast Neoplasms, Nuclear Proteins, Adenocarcinoma, Receptors, Estrogen, Membrane Glycoproteins, Neoplasm Proteins, Paired Box Transcription Factors, Tumor Cells, Cultured, Ovarian Neoplasms, Adenocarcinoma, Clear Cell, Carcinoma, Papillary, Cation Transport Proteins, Ephrin-B1, GATA3 Transcription Factor, GPI-Linked Proteins Journal Mol Biol Cell Volume 14 Issue 11 Pages 4376-86 Date Published 11/2003 Alternate Journal Mol. Biol. Cell Google ScholarBibTeXEndNote X3 XML