A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens.

Publication Year
2012

Type

Journal Article
Abstract

Because off-target effects hamper interpretation and validation of RNAi screen data, we developed a bioinformatics method, genome-wide enrichment of seed sequence matches (GESS), to identify candidate off-targeted transcripts in primary screening data. GESS analysis revealed a prominent off-targeted transcript in several screens, including MAD2 (MAD2L1) in a screen for genes required for the spindle assembly checkpoint. GESS analysis results can enhance the validation rate in RNAi screens.

Journal
Nature methods
Volume
9
Issue
4
Pages
363-6
Date Published
02/2012
ISSN Number
1548-7105
Alternate Journal
Nat Methods
PMCID
PMC3482495
PMID
22343343