Accumulation of recessive lethal mutations in Saccharomyces cerevisiae mlh1 mismatch repair mutants is not associated with gross chromosomal rearrangements. Author Julie Heck, David Gresham, David Botstein, Eric Alani Publication Year 2006 Type Journal Article Abstract We examined mismatch repair (MMR)-defective diploid strains of budding yeast grown for approximately 160 generations to determine whether decreases in spore viability due to the uncovering of recessive lethal mutations correlated with an increase in gross chromosomal rearrangements (GCRs). No GCRs were detected despite dramatic decreases in spore viability, suggesting that frameshift and/or other unrepaired DNA replication lesions play a greater role than chromosomal instability in decreasing viability in MMR-defective strains. Keywords Mutation, Saccharomyces cerevisiae, Cell Survival, Models, Biological, Fungal Proteins, Adaptor Proteins, Signal Transducing, Chromosome Aberrations, Nucleic Acid Hybridization, Saccharomyces cerevisiae Proteins, Genes, Lethal, Probability, Genes, Recessive, Gene Rearrangement, Base Pair Mismatch, Frameshift Mutation Journal Genetics Volume 174 Issue 1 Pages 519-23 Date Published 09/2006 Alternate Journal Genetics Google ScholarBibTeXEndNote X3 XML
