TitleRapid Rates of Pol II Elongation in the Drosophila Embryo.
Publication TypeJournal Article
Year of Publication2017
AuthorsFukaya, T, Lim, B, Levine, M
JournalCurr Biol
Date Published2017 May 08

Elongation of RNA polymerase II (Pol II) is thought to be an important mechanism for regulating gene expression [1]. We measured the first wave of de novo transcription in living Drosophila embryos using dual-fluorescence detection of nascent transcripts containing 5' MS2 and 3' PP7 RNA stem loops. Pol II elongation rates of 2.4-3.0 kb/min were observed, approximately twice as fast as earlier estimates [2-6]. The revised rates permit substantial levels of zygotic gene activity prior to the mid-blastula transition. We also provide evidence that variable rates of elongation are not a significant source of differential gene activity, suggesting that transcription initiation and Pol II release are the key determinants of gene control in development.

Alternate JournalCurr. Biol.
PubMed ID28457866
PubMed Central IDPMC5665007
Grant ListF32 GM122186 / GM / NIGMS NIH HHS / United States
R01 GM034431 / GM / NIGMS NIH HHS / United States
R35 GM118147 / GM / NIGMS NIH HHS / United States
U01 EB021239 / EB / NIBIB NIH HHS / United States