TitlePhysical linkage of two mammalian imprinted genes, H19 and insulin-like growth factor 2.
Publication TypeJournal Article
Year of Publication1992
AuthorsZemel, S, Bartolomei, MS, Tilghman, SM
JournalNat Genet
Volume2
Issue1
Pagination61-5
Date Published1992 Sep
KeywordsAnimals, Chromosomes, Fungal, Cloning, Molecular, Female, Gene Expression, Gene Library, Genetic Linkage, Humans, Insulin-Like Growth Factor II, Male, Mice, Restriction Mapping, Sex Characteristics
Abstract

Parental imprinting is a phenomenon in mammals whereby the maternal and paternal alleles of a gene are differentially expressed. Three murine genes have been shown to display this type of allele-specific expression. Two of them, insulin-like growth factor-2 (Igf-2) and H19, map to the distal end of mouse chromosome 7, but are imprinted in opposite directions. Pulsed-field gel electrophoresis and large-fragment DNA cloning were utilized to establish a physical map that includes H19 and Igf-2. Igf-2 lies approximately 90 kilobases of DNA 5' to H19, in the same transcriptional orientation. This physical proximity is conserved in humans, based on pulsed-field gel analysis. We conclude that H19 and Igf-2 constitute an imprinted domain.

Alternate JournalNat. Genet.