TitlePhysical linkage of two mammalian imprinted genes, H19 and insulin-like growth factor 2.
Publication TypeJournal Article
Year of Publication1992
AuthorsZemel, S, Bartolomei, MS, Tilghman, SM
JournalNat Genet
Date Published1992 Sep
KeywordsAnimals, Chromosomes, Fungal, Cloning, Molecular, Female, Gene Expression, Gene Library, Genetic Linkage, Humans, Insulin-Like Growth Factor II, Male, Mice, Restriction Mapping, Sex Characteristics

Parental imprinting is a phenomenon in mammals whereby the maternal and paternal alleles of a gene are differentially expressed. Three murine genes have been shown to display this type of allele-specific expression. Two of them, insulin-like growth factor-2 (Igf-2) and H19, map to the distal end of mouse chromosome 7, but are imprinted in opposite directions. Pulsed-field gel electrophoresis and large-fragment DNA cloning were utilized to establish a physical map that includes H19 and Igf-2. Igf-2 lies approximately 90 kilobases of DNA 5' to H19, in the same transcriptional orientation. This physical proximity is conserved in humans, based on pulsed-field gel analysis. We conclude that H19 and Igf-2 constitute an imprinted domain.

Alternate JournalNat. Genet.