TitleA Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis.
Publication TypeJournal Article
Year of Publication2017
AuthorsBartlett, TM, Bratton, BP, Duvshani, A, Miguel, A, Sheng, Y, Martin, NR, Nguyen, JP, Persat, A, Desmarais, SM, VanNieuwenhze, MS, Huang, KCasey, Zhu, J, Shaevitz, JW, Gitai, Z
Date Published2017 Jan 12
KeywordsAmino Acid Sequence, Animals, Bacterial Proteins, Locomotion, Mice, Peptidoglycan, Periplasm, Sequence Alignment, Vibrio cholerae, Virulence

Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. The mechanism and function of this curvature were previously unknown. Here, we identify and characterize CrvA, the first curvature determinant in V. cholerae. CrvA self-assembles into filaments at the inner face of cell curvature. Unlike traditional cytoskeletons, CrvA localizes to the periplasm and thus can be considered a periskeletal element. To quantify how curvature forms, we developed QuASAR (quantitative analysis of sacculus architecture remodeling), which measures subcellular peptidoglycan dynamics. QuASAR reveals that CrvA asymmetrically patterns peptidoglycan insertion rather than removal, causing more material insertions into the outer face than the inner face. Furthermore, crvA is quorum regulated, and CrvA-dependent curvature increases at high cell density. Finally, we demonstrate that CrvA promotes motility in hydrogels and confers an advantage in host colonization and pathogenesis.

Alternate JournalCell
PubMed ID28086090
PubMed Central IDPMC5287421
Grant ListDP1 AI124669 / AI / NIAID NIH HHS / United States
P50 GM071508 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
R01 GM107384 / GM / NIGMS NIH HHS / United States
R01 AI120489 / AI / NIAID NIH HHS / United States
DP2 OD006466 / OD / NIH HHS / United States
R01 GM113172 / GM / NIGMS NIH HHS / United States