Monitoring protein synthesis one codon at a time through ribosome profiling
Monday, November 14, 2011 - 4:15pm to 5:15pm
Carl Icahn Lab 101
Quantitative & Computational Biology
Lewis-Sigler Institute

The ability to sequence genomes has far outstripped approaches for deciphering the information they encode. We have developed a suite of techniques based on ribosome profiling (deep sequencing of ribosome protected fragments) that dramatically expand our ability to follow translation in vivo. I will present recent applications of our ribosome profiling approach including the following: (1) Development of ribosome profiling protocols for a wide variety of eukaryotic and prokaryotic organisms. (2) Uses of ribosome profiling to globally monitor when chaperones, targeting factors or processing enzymes engage nascent chains. (3) Deciphering the driving force and biological consequences underlying the choice of synonymous codons.

The Weissman Lab at UCSF.