The focus of our research is to develop ways of tracking ions, molecules, and proteins in cells using fluorescence microscopy. We have created genetically encoded sensors that utilize FRET to track calcium and zinc ions in cells. We utilize these sensors to study metal homeostasis and define dynamics during signaling processes. We have also developed ways of imaging bacterial protein secretion so we can monitor pathogenic proteins in host cells. Using this methodology we have identified bacterial proteins that interact with and perturb vesicle trafficking in host cells.
Developing probes for live cell imaging of cell signaling and host-pathogen interactions
Monday, April 4, 2011 - 4:15pm to 5:15pm
Carl Icahn Lab 101
Quantitative & Computational Biology