Abstract: RNA is the fundamental information transfer system in the cell. Its synthesis, localization, translation and transport ultimately determine when and where the protein product is made. We develop single molecule imaging and spectroscopy technology to study RNA in vivo. We track endogenous mRNAs in live neurons and follow their localization in response to synaptic stimulation. Recently we established a single molecule translation assay (SINAPS) to directly measure the translation initiation, elongation speed and spatial location in live cells. We are investigating how synaptic stimulation influences RNA localization and translation in dendrites, which plays an important role in synaptic plasticity, memory and learning.
About Dr. Wu and his research:
Seeing is believing. Bin Wu’s primary research interest is visualizing and quantifying biological events as they happen inside live cells. He uses single molecule imaging and spectroscopy technology, combined with theoretical modeling to extract dynamic biological information. Currently, the lab is working on how cells regulate gene expression in subcellular compartments. To do that, he tracks the birth, trafficking, translation, and decay of single mRNAs in live cells and tissues.
Dr. Wu is Assistant Professor in the Department of Biophysics and Biophysical Chemistry at the Johns Hopkins University School of Medicine. He received the 2011 Gregorio Weber International Prize in Biological Fluorescence and is a 2017 Pew Biomedical Scholar.
Wu Lab website: http://wu.med.jhmi.edu/research/