TitleThe Neuronal Kinesin UNC-104/KIF1A Is a Key Regulator of Synaptic Aging and Insulin Signaling-Regulated Memory.
Publication TypeJournal Article
Year of Publication2016
AuthorsLi, L-B, Lei, H, Arey, RN, Li, P, Liu, J, Murphy, CT, Xu, XZShawn, Shen, K
JournalCurr Biol
Date Published2016 Mar 07
KeywordsAging, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Insulin, Memory, Nerve Tissue Proteins, Signal Transduction, Synapses

Aging is the greatest risk factor for a number of neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. Furthermore, normal aging is associated with a decline in sensory, motor, and cognitive functions. Emerging evidence suggests that synapse alterations, rather than neuronal cell death, are the causes of neuronal dysfunctions in normal aging and in early stages of neurodegenerative diseases. However, little is known about the mechanisms underlying age-related synaptic decline. Here, we uncover a surprising role of the anterograde molecular motor UNC-104/KIF1A as a key regulator of neural circuit deterioration in aging C. elegans. Through analyses of synapse protein localization, synaptic transmission, and animal behaviors, we find that reduced function of UNC-104 accelerates motor circuit dysfunction with age, whereas upregulation of UNC-104 significantly improves motor function at advanced ages and also mildly extends lifespan. In addition, UNC-104-overexpressing animals outperform wild-type controls in associative learning and memory tests. Further genetic analyses suggest that UNC-104 functions downstream of the DAF-2-signaling pathway and is regulated by the FOXO transcription factor DAF-16, which contributes to the effects of DAF-2 in neuronal aging. Together, our cellular, electrophysiological, and behavioral analyses highlight the importance of axonal transport in the maintenance of synaptic structural integrity and function during aging and raise the possibility of targeting kinesins to slow age-related neural circuit dysfunction.

Alternate JournalCurr. Biol.
PubMed ID26877087
PubMed Central IDPMC4783184
Grant ListF32 AG046106 / AG / NIA NIH HHS / United States
R01 AG034446 / AG / NIA NIH HHS / United States
R01 AG048072 / AG / NIA NIH HHS / United States
/ / Howard Hughes Medical Institute / United States