TitleMitosis-associated repression in development.
Publication TypeJournal Article
Year of Publication2016
AuthorsEsposito, E, Lim, B, Guessous, G, Falahati, H, Levine, M
JournalGenes Dev
Date Published2016 Jul 1

Transcriptional repression is a pervasive feature of animal development. Here, we employ live-imaging methods to visualize the Snail repressor, which establishes the boundary between the presumptive mesoderm and neurogenic ectoderm of early Drosophila embryos. Snail target enhancers were attached to an MS2 reporter gene, permitting detection of nascent transcripts in living embryos. The transgenes exhibit initially broad patterns of transcription but are refined by repression in the mesoderm following mitosis. These observations reveal a correlation between mitotic silencing and Snail repression. We propose that mitosis and other inherent discontinuities in transcription boost the activities of sequence-specific repressors, such as Snail.

Alternate JournalGenes Dev.
PubMed ID27401553
PubMed Central IDPMC4949323
Grant ListR01 GM046638 / GM / NIGMS NIH HHS / United States
R37 GM046638 / GM / NIGMS NIH HHS / United States