TitleMass spectrometry-based metabolomics of yeast.
Publication TypeJournal Article
Year of Publication2010
AuthorsCrutchfield, CA, Lu, W, Melamud, E, Rabinowitz, JD
JournalMethods Enzymol
Date Published2010
KeywordsChromatography, Liquid, Mass Spectrometry, Metabolomics, Yeasts

Driven by the advent of metabolomics, recent years have seen renewed interest in the investigation of yeast metabolism. Here we provide a practical guide to metabolomic analysis of yeast using liquid chromatography-mass spectrometry (LC-MS). We begin with background on LC-MS and its utility in studying yeast metabolism. We then describe key issues involved at each step of a typical yeast metabolomics experiment: in experimental design, cell culture, metabolite extraction, LC-MS, and data processing and analysis. Throughout, we highlight interdependencies between the steps that are relevant to developing an integrated workflow which effectively leverages LC-MS to reveal yeast biology.

Alternate JournalMeth. Enzymol.