Research Specialist I/II Position - Requisition# 2019-10784
Abstract: The adoptive transfer of T cells expressing chimeric antigen receptors (CARs) has demonstrated clinical efficacy in the treatment of advanced cancers, with anti-CD19 CAR-T cells achieving up to 90% complete remission among patients with relapsed B-cell malignancies. However, challenges such as antigen escape and immunosuppression limit the long-term efficacy of adoptive T-cell therapy.
About Dr. McKnight:
Abstract: Proteomics and other omic approaches constitute a powerful and versatile toolkit for probing biological systems. Encompassing many layers of biological information, multi-omic data holistically describe a living system and its response to perturbations. Mitochondria – central hubs of cellular metabolism – are particularly amiable to multi-omic profiling, as myriad metabolites, lipids, and proteins co-function in these organelles and jointly orchestrate responses to various changes.
Abstract: With the advent of fast and cheap genome sequencing, functional analysis is now a bottleneck for understanding human genetics and implementing personalized medicine. We are leveraging the awesome power of yeast genetics to create high throughput methods for testing the function of hundreds or thousands of human genetic variants simultaneously. I'll discuss two such assays we've developed, one for activity of cytochrome P450s, which are important for drug metabolism, and a second for mutation rate, which is important for cancer risk.
Abstract: During mitosis in eukaryotes, each genomic linear DNA is folded into an individualized chromosome with characteristic shape and size, which differ among cell types and species. While prevailing models suggest that chromosomes are shaped through DNA loop formation, mediated by chromosome-associated ATPases, condensins and DNA topoisomerase II, it remains unclear how these enzymes act on nucleosomes, the fundamental unit of chromatin where DNA wraps around histones. I will present our study on a new role of histones on controlling mitotic chromosome structure and function.