TitleKinetic flux profiling for quantitation of cellular metabolic fluxes.
Publication TypeJournal Article
Year of Publication2008
AuthorsYuan, J, Bennett, BD, Rabinowitz, JD
JournalNat Protoc
Date Published2008
KeywordsCarbon, Carbon Isotopes, Cell Culture Techniques, Chromatography, High Pressure Liquid, Escherichia coli, Kinetics, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry

This protocol enables quantitation of metabolic fluxes in cultured cells. Measurements are based on the kinetics of cellular incorporation of stable isotope from nutrient into downstream metabolites. At multiple time points, after cells are rapidly switched from unlabeled to isotope-labeled nutrient, metabolism is quenched, metabolites are extracted and the extract is analyzed by chromatography-mass spectrometry. Resulting plots of unlabeled compound versus time follow variants of exponential decay, with the flux equal to the decay rate multiplied by the intracellular metabolite concentration. Because labeling is typically fast (t(1/2)

Alternate JournalNat Protoc