TitleKinetic flux profiling of nitrogen assimilation in Escherichia coli.
Publication TypeJournal Article
Year of Publication2006
AuthorsYuan, J, Fowler, WU, Kimball, E, Lu, W, Rabinowitz, JD
JournalNat Chem Biol
Date Published2006 Oct
KeywordsEscherichia coli, Kinetics, Nitrogen, Time Factors

We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.

Alternate JournalNat. Chem. Biol.