TitleImmunity regulatory DNAs share common organizational features in Drosophila.
Publication TypeJournal Article
Year of Publication2004
AuthorsSenger, K, Armstrong, GW, Rowell, WJ, Kwan, JM, Markstein, M, Levine, M
JournalMol Cell
Date Published2004 Jan 16
KeywordsAnimals, Animals, Genetically Modified, Binding Sites, Cells, Cultured, Consensus Sequence, Drosophila, Drosophila Proteins, Embryo, Nonmammalian, Enhancer Elements, Genetic, Fat Body, Gene Expression Regulation, Genes, Insect, Immunity, Larva, Models, Genetic, Regulatory Sequences, Nucleic Acid, Sequence Deletion, Transcription Factors, Transcriptional Activation

Infection results in the rapid activation of immunity genes in the Drosophila fat body. Two classes of transcription factors have been implicated in this process: the REL-containing proteins, Dorsal, Dif, and Relish, and the GATA factor Serpent. Here we present evidence that REL-GATA synergy plays a pervasive role in the immune response. SELEX assays identified consensus binding sites that permitted the characterization of several immunity regulatory DNAs. The distribution of REL and GATA sites within these DNAs suggests that most or all fat-specific immunity genes contain a common organization of regulatory elements: closely linked REL and GATA binding sites positioned in the same orientation and located near the transcription start site. Aspects of this "regulatory code" are essential for the immune response. These results suggest that immunity regulatory DNAs contain constrained organizational features, which may be a general property of eukaryotic enhancers.

Alternate JournalMol. Cell