TitleIdentifying decomposition products in extracts of cellular metabolites.
Publication TypeJournal Article
Year of Publication2006
AuthorsKimball, E, Rabinowitz, JD
JournalAnal Biochem
Date Published2006 Nov 15
KeywordsChromatography, Liquid, Escherichia coli, Mass Spectrometry, Methanol, Reference Standards, Water

Most methods of analyzing intracellular metabolites require extraction of metabolites from the cells. A concern in these methods is underestimation of metabolite levels due to incomplete extraction. In comparing extraction methods, then, it would seem that the best method for extracting a particular metabolite is the one that gives the largest yield. In extracting Escherichia coli with different methanol:water mixtures, we observed that >or=50% water gave an increased yield of nucleosides and bases compared with

Alternate JournalAnal. Biochem.