TitleOn the GFP-based analysis of dynamic concentration profiles.
Publication TypeJournal Article
Year of Publication2014
AuthorsBerezhkovskii, AM, Shvartsman, SY
JournalBiophys J
Date Published2014 Feb 4
KeywordsAnimals, Drosophila, Fluorescence, Green Fluorescent Proteins, Models, Biological, Morphogenesis

Studies with GFP-tagged proteins can be used to investigate the dynamics of concentration profiles of regulatory proteins in cells and tissues. Analysis of these experiments must account for the finite rate with which the GFP-tagged proteins mature to the fluorescent state. Toward this end, we present an analytical framework that provides an explicit connection between the apparent kinetics of concentration gradients and the rates of GFP maturation.

Alternate JournalBiophys. J.