Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.

Title	Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.
Publication Type	Journal Article
Year of Publication	1992
Authors	Rossi, JM, Burke, DT, Leung, JC, Koos, DS, Chen, H, Tilghman, SM
Journal	Proc Natl Acad Sci U S A
Volume	89
Issue	6
Pagination	2456-60
Date Published	1992 Mar 15
Keywords	Animals, Base Sequence, Chromosome Mapping, Chromosomes, Fungal, DNA, Exons, Female, Gene Library, Genetic Markers, Genome, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Oligodeoxyribonucleotides, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Saccharomyces cerevisiae, Spleen
Abstract	A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.
Alternate Journal	Proc. Natl. Acad. Sci. U.S.A.