|Title||Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA microarray.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Gresham, D, Ruderfer, DM, Pratt, SC, Schacherer, J, Dunham, MJ, Botstein, D, Kruglyak, L|
|Date Published||2006 Mar 31|
|Keywords||Directed Molecular Evolution, Genes, Fungal, Genome, Fungal, Genomics, Mutation, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Phenotype, Point Mutation, Polymorphism, Single Nucleotide, Saccharomyces cerevisiae, Sequence Deletion, Suppression, Genetic|
A central challenge of genomics is to detect, simply and inexpensively, all differences in sequence among the genomes of individual members of a species. We devised a system to detect all single-nucleotide differences between genomes with the use of data from a single hybridization to a whole-genome DNA microarray. This allowed us to detect a variety of spontaneous single-base pair substitutions, insertions, and deletions, and most (>90%) of the approximately 30,000 known single-nucleotide polymorphisms between two Saccharomyces cerevisiae strains. We applied this approach to elucidate the genetic basis of phenotypic variants and to identify the small number of single-base pair changes accumulated during experimental evolution of yeast.