TitleGenome-wide detection of polymorphisms at nucleotide resolution with a single DNA microarray.
Publication TypeJournal Article
Year of Publication2006
AuthorsGresham, D, Ruderfer, DM, Pratt, SC, Schacherer, J, Dunham, MJ, Botstein, D, Kruglyak, L
Date Published2006 Mar 31
KeywordsDirected Molecular Evolution, Genes, Fungal, Genome, Fungal, Genomics, Mutation, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Phenotype, Point Mutation, Polymorphism, Single Nucleotide, Saccharomyces cerevisiae, Sequence Deletion, Suppression, Genetic

A central challenge of genomics is to detect, simply and inexpensively, all differences in sequence among the genomes of individual members of a species. We devised a system to detect all single-nucleotide differences between genomes with the use of data from a single hybridization to a whole-genome DNA microarray. This allowed us to detect a variety of spontaneous single-base pair substitutions, insertions, and deletions, and most (>90%) of the approximately 30,000 known single-nucleotide polymorphisms between two Saccharomyces cerevisiae strains. We applied this approach to elucidate the genetic basis of phenotypic variants and to identify the small number of single-base pair changes accumulated during experimental evolution of yeast.

Alternate JournalScience