Overview

The Lewis-Sigler Institute for Integrative Genomics houses the Genomics Core Facility which provides researchers with access to “Next Generation” high-throughput DNA sequencing technologies. The staff provide consultation on experimental design, library preparation, and data analysis. The Genomics Core Facility works closely with the Bioinformatics staff in the Lewis-Sigler Institute to provide researchers with computing power and consulting services to analyze sequencing data.

The facility currently houses an Illumina NovaSeq 6000 and MiSeq, and is staffed to provide full sequencing service for researchers NGS experiments.  In addition to sequencing some of the other services provided include:

  • Library preparation for DNA samples: genomic DNA, ChIP-seq, amplicon, linked reads on long DNA using 10X Genomics Chromium system

  • Library preparation for RNA samples:  strand-specific RNA-seq, microRNA and small RNA-seq,  rRNA-depletion, SMART-seq for low input RNA, single cell RNA-seq using 10X Genomics Chromium system

  • DNA Size selection and clean up through BluePippin or Ampure XP Beads

  • DNA and RNA sample QC by Bioanalyzer or TapeStation

The Facility also has several pieces of ancillary instrumentation that researches have access to upon initial training.  These instruments include:  

The entire lab is ozone free protecting the dye integrity of labeled samples.