Overview
The Lewis-Sigler Institute for Integrative Genomics houses the Genomics Core Facility which provides researchers with access to “Next Generation” high-throughput DNA sequencing technologies. The staff provide consultation on experimental design, library preparation, and data analysis. The Genomics Core Facility works closely with the Bioinformatics staff in the Lewis-Sigler Institute to provide researchers with computing power and consulting services to analyze sequencing data.
The facility currently houses an Illumina NovaSeq 6000 and MiSeq, and is staffed to provide full sequencing service for researchers NGS experiments. In addition to sequencing some of the other services provided include:
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Library preparation for DNA samples: genomic DNA, ChIP-seq, amplicon, linked reads on long DNA using 10X Genomics Chromium system
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Library preparation for RNA samples: strand-specific RNA-seq, microRNA and small RNA-seq, rRNA-depletion, SMART-seq for low input RNA, single cell RNA-seq using 10X Genomics Chromium system
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DNA Size selection and clean up through BluePippin or Ampure XP Beads
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DNA and RNA sample QC by Bioanalyzer or TapeStation
The Facility also has several pieces of ancillary instrumentation that researches have access to upon initial training. These instruments include:
- Qubit 2.0
- Nanodrop 1000
- Moxi Go II flow cytometer
- Agilent Bioanalyzer
- Agilent TapeStation 4200
- Covaris LE 220
- BluePippin DNA size selection system
- Biotek Synergy Mx plate reader
- Real Time PCR using ABI 7900 HT (96 well) or ViiA7 (96 or 384 well)
- Bio-Rad QX200 AutoDG Droplet Digital PCR system
- Agilent Microarray Scanner with Surescan High-Resolution Technology
- BiomekFX Liquid Handling Robot
- Kingfisher Flex for DNA or RNA extraction
- Beckman-Coulter Avanti-JE High Capacity Centrifuge
The entire lab is ozone free protecting the dye integrity of labeled samples.