In Light Sheet fluorescence microscopy the samples are illuminated from the side by a thin sheet of light positioned exactly at the focal plane of the imaging objective. Illumination light is confined only to a thin section that is being imaged, avoiding the unncessary and potentially photo-toxic illumination of other areas of the sample. Use of high-end (82% Peak Quantum Efficiency) sCMOS cameras allow for sensitive detection and resolution. This approach considerably reduces the out-of-focus fluorescence compared to wide-field epi-fluorescence, therefore improving contrast, and reducing photobleaching. The method is fast as it does not rely on raster-scanning a laser beam and is readily compatible with large field of views.
In the facility, we have a built an advanced Light Sheet Microscope with
Dual-Sided Excitation and Dual-Sided Detection with
2 sCMOS Cameras (latest, 82% Quantum Efficiency)
Fast Piezo Stage for XY Sample Positioning with 1500 microns of total travel
Fast Rotation Stage (up to 720 deg s-1)
Variety of Excitation and Objective Lenses (including 2 Nikon 25x 1.1 NA)
Two camera splitters for fast dual-color imaging.
Full set of 405nm, 488nm, 514nm, 561nm, 594nm, and 647nm laser lines that are directly modulated by National Instruments DAQ hardware for fast, multi-color, live imaging.
Local machine with 38TB of starge and 10Gb local network.