Light Sheet Fluorescence Excitation

In Light Sheet fluorescence microscopy the samples are illuminated from the side by a thin sheet of light positioned exactly at the focal plane of the imaging objective.  Illumination light is confined only to a thin section that is being imaged, avoiding the unncessary and potentially photo-toxic illumination of other areas of the sample.  Use of high-end (82% Peak Quantum Efficiency) sCMOS cameras allow for sensitive detection and resolution. This approach considerably reduces the out-of-focus fluorescence compared to wide-field epi-fluorescence, therefore improving contrast, and reducing photobleaching. The method is fast as it does not rely on raster-scanning a laser beam  and is readily compatible with large field of views.

In the facility, we have a built an advanced Light Sheet Microscope with

Dual-Sided Excitation and Dual-Sided Detection with

2 sCMOS Cameras (latest, 82% Quantum Efficiency)

Fast Piezo Stage for XY Sample Positioning with 1500 microns of total travel

Fast Rotation Stage (up to 720 deg s-1)

Variety of Excitation and Objective Lenses (including 2 Nikon 25x 1.1 NA)

Two camera splitters for fast dual-color imaging.

Full set of 405nm, 488nm, 514nm, 561nm, 594nm, and 647nm laser lines that are directly modulated by National Instruments DAQ hardware for fast, multi-color, live imaging.

Local machine with 38TB of starge and 10Gb local network.