TitleDynamic structures in Escherichia coli: spontaneous formation of MinE rings and MinD polar zones.
Publication TypeJournal Article
Year of Publication2003
AuthorsHuang, KCasey, Meir, Y, Wingreen, NS
JournalProc Natl Acad Sci U S A
Date Published2003 Oct 28
KeywordsAdenosine Triphosphatases, Adenosine Triphosphate, Cell Cycle Proteins, Cell Polarity, Escherichia coli, Escherichia coli Proteins, Hydrolysis, Oscillometry, Protein Conformation

In Escherichia coli, division site selection is regulated in part by the Min-protein system. Oscillations of the Min proteins from pole to pole every approximately 40 sec have been revealed by in vivo studies of GFP fusions. The dynamic oscillatory structures produced by the Min proteins, including a ring of MinE protein, compact polar zones of MinD, and zebra-striped oscillations in filamentous cells, remain unexplained. We show that the Min oscillations, including mutant phenotypes, can be accounted for by in vitro-observed interactions involving MinD and MinE, with a crucial role played by the rate of nucleotide exchange. Recent discoveries suggest that protein oscillations may play a general role in proper chromosome and plasmid partitioning.

Alternate JournalProc. Natl. Acad. Sci. U.S.A.