|Title||The Dlk1 and Gtl2 genes are linked and reciprocally imprinted.|
|Publication Type||Journal Article|
|Year of Publication||2000|
|Authors||Schmidt, JV, Matteson, PG, Jones, BK, Guan, XJ, Tilghman, SM|
|Date Published||2000 Aug 15|
|Keywords||Animals, Base Sequence, Chromosome Mapping, DNA Methylation, DNA Primers, Female, Gene Expression Regulation, Developmental, Genetic Linkage, Genomic Imprinting, Muridae, Peromyscus, Reverse Transcriptase Polymerase Chain Reaction|
Genes subject to genomic imprinting exist in large chromosomal domains, probably reflecting coordinate regulation of the genes within a cluster. Such regulation has been demonstrated for the H19, Igf2, and Ins2 genes that share a bifunctional imprinting control region. We have identified the Dlk1 gene as a new imprinted gene that is paternally expressed. Furthermore, we show that Dlk1 is tightly linked to the maternally expressed Gtl2 gene. Dlk1 and Gtl2 are coexpressed and respond in a reciprocal manner to loss of DNA methylation. These genes are likely to represent a new example of coordinated imprinting of linked genes.
|Alternate Journal||Genes Dev.|