TitleDiscovery and Functional Characterization of a Yeast Sugar Alcohol Phosphatase.
Publication TypeJournal Article
Year of Publication2018
AuthorsXu, Y-F, Lu, W, Chen, JC, Johnson, SA, Gibney, PA, Thomas, DG, Brown, G, May, AL, Campagna, SR, Yakunin, AF, Botstein, D, Rabinowitz, JD
JournalACS Chem Biol
Volume13
Issue10
Pagination3011-3020
Date Published2018 10 19
ISSN1554-8937
KeywordsGene Deletion, Glucose-6-Phosphate Isomerase, Hydrolysis, Phosphoric Monoester Hydrolases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sugar Phosphates
Abstract

Sugar alcohols (polyols) exist widely in nature. While some specific sugar alcohol phosphatases are known, there is no known phosphatase for some important sugar alcohols (e.g., sorbitol-6-phosphate). Using liquid chromatography-mass spectrometry-based metabolomics, we screened yeast strains with putative phosphatases of unknown function deleted. We show that the yeast gene YNL010W, which has close homologues in all fungi species and some plants, encodes a sugar alcohol phosphatase. We term this enzyme, which hydrolyzes sorbitol-6-phosphate, ribitol-5-phosphate, and (d)-glycerol-3-phosphate, polyol phosphatase 1 or PYP1. Polyol phosphates are structural analogs of the enediol intermediate of phosphoglucose isomerase (Pgi). We find that sorbitol-6-phosphate and ribitol-5-phosphate inhibit Pgi and that Pyp1 activity is important for yeast to maintain Pgi activity in the presence of environmental sugar alcohols. Pyp1 expression is strongly positively correlated with yeast growth rate, presumably because faster growth requires greater glycolytic and accordingly Pgi flux. Thus, yeast express the previously uncharacterized enzyme Pyp1 to prevent inhibition of glycolysis by sugar alcohol phosphates. Pyp1 may be useful for engineering sugar alcohol production.

DOI10.1021/acschembio.8b00804
Alternate JournalACS Chem. Biol.
PubMed ID30240188
PubMed Central IDPMC6466636
Grant ListP50 GM071508 / GM / NIGMS NIH HHS / United States
R01 CA163591 / CA / NCI NIH HHS / United States
R50 CA211437 / CA / NCI NIH HHS / United States