TitleControl of intercalation is cell-autonomous in the notochord of Ciona intestinalis.
Publication TypeJournal Article
Year of Publication2002
AuthorsKeys, DN, Levine, M, Harland, RM, Wallingford, JB
JournalDev Biol
Date Published2002 Jun 15
KeywordsAnimals, Animals, Genetically Modified, Base Sequence, Ciona intestinalis, DNA Primers, Morphogenesis, Mutation, Notochord

Dishevelled signaling plays a critical role in the control of cell intercalation during convergent extension in vertebrates. This study presents evidence that Dishevelled serves a similar function in the Ciona notochord. Embryos transgenic for mutant Dishevelled fail to elongate their tails, and notochord cells fail to intercalate, though notochord cell fates are unaffected. Analysis of mosaic transgenics revealed that the effects of mutant Dishevelled on notochord intercalation are cell-autonomous in Ciona, though such defects have nonautonomous effects in Xenopus. Furthermore, our data indicate that notochord cell intercalation in Ciona does not require the progressive signals which coordinate cell intercalation in the Xenopus notochord, highlighting an important difference in how mediolateral cell intercalation is controlled in the two animals. Finally, this study establishes the Ciona embryo as an effective in vivo system for the study of the molecular control of morphogenetic cell movements in chordates.

Alternate JournalDev. Biol.