|Title||The Bicoid gradient is shaped independently of nuclei.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Grimm, O, Wieschaus, E|
|Date Published||2010 Sep 1|
|Keywords||Active Transport, Cell Nucleus, Animals, Animals, Genetically Modified, Body Patterning, Cell Nucleus, Drosophila melanogaster, Genes, Insect, Homeodomain Proteins, Kinetics, Mutant Proteins, Recombinant Proteins, Sequence Deletion, Trans-Activators|
Morphogen gradients provide embryos with positional information, yet how they form is not understood. Binding of the morphogen to receptors could affect the formation of the morphogen gradient, in particular if the number of morphogen binding sites changes with time. For morphogens that function as transcription factors, the final distribution can be heavily influenced by the number of nuclear binding sites. Here, we have addressed the role of the increasing number of nuclei during the formation of the Bicoid gradient in embryos of Drosophila melanogaster. Deletion of a short stretch of sequence in Bicoid impairs its nuclear accumulation. This effect is due to a approximately 4-fold decrease in nuclear import rate and a approximately 2-fold reduction in nuclear residence time compared with the wild-type protein. Surprisingly, the shape of the resulting anterior-posterior gradient as well as the centre-surface distribution are indistinguishable from those of the normal gradient. This suggests that nuclei do not shape the Bicoid gradient but instead function solely during its interpretation.