@article{2533,
  keywords = {Saccharomyces cerevisiae, Gene Regulatory Networks, Saccharomyces cerevisiae Proteins, Genes, Fungal, Epistasis, Genetic, Genetic Pleiotropy, Genes, Essential},
  author = {Michael Costanzo and Benjamin VanderSluis and Elizabeth Koch and Anastasia Baryshnikova and Carles Pons and Guihong Tan and Wen Wang and Matej Usaj and Julia Hanchard and Susan Lee and Vicent Pelechano and Erin Styles and Maximilian Billmann and Jolanda van Leeuwen and Nydia van Dyk and Zhen-Yuan Lin and Elena Kuzmin and Justin Nelson and Jeff Piotrowski and Tharan Srikumar and Sondra Bahr and Yiqun Chen and Raamesh Deshpande and Christoph Kurat and Sheena Li and Zhijian Li and Mojca Usaj and Hiroki Okada and Natasha Pascoe and Bryan-Joseph San Luis and Sara Sharifpoor and Emira Shuteriqi and Scott Simpkins and Jamie Snider and Harsha Suresh and Yizhao Tan and Hongwei Zhu and Noel Malod-Dognin and Vuk Janjic and Natasa Przulj and Olga Troyanskaya and Igor Stagljar and Tian Xia and Yoshikazu Ohya and Anne-Claude Gingras and Brian Raught and Michael Boutros and Lars Steinmetz and Claire Moore and Adam Rosebrock and Amy Caudy and Chad Myers and Brenda Andrews and Charles Boone},
  title = {A global genetic interaction network maps a wiring diagram of cellular function.},
  abstract = {<p>We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to protein complexes and pathways, biological processes, and cellular compartments. Negative interactions connected functionally related genes, mapped core bioprocesses, and identified pleiotropic genes, whereas positive interactions often mapped general regulatory connections among gene pairs, rather than shared functionality. The global network illustrates how coherent sets of genetic interactions connect protein complex and pathway modules to map a functional wiring diagram of the cell.</p>
},
  year = {2016},
  journal = {Science},
  volume = {353},
  month = {09/2016},
  issn = {1095-9203},
  doi = {10.1126/science.aaf1420},
  language = {eng},
}