@article{2255,
  keywords = {Animals, Drosophila, Larva, Microtubules, Cell Nucleus, Kinetics, Biological Transport, Insect Proteins, Mutagenesis, Kinesin, Eye, Lipid Metabolism, Intracellular Membranes},
  author = {M Welte and S Gross and M Postner and S Block and E Wieschaus},
  title = {Developmental regulation of vesicle transport in Drosophila embryos: forces and kinetics.},
  abstract = {<p>In Drosophila embryos, microtubules oriented along apical-basal directions support saltatory vesicle movement. Vesicle traffic includes lipid droplets whose distribution shifts twice during early embryogenesis. Using microscopy, optical tweezers, and a novel squashed-mount embryo preparation, we tracked single droplets and measured the forces these generated. Droplet stalling forces change developmentally, in a roughly quantized fashion, consistent with variation in the number of active motors. We characterized a mutation, klarsicht, that affects droplet transport. Klar+ facilitates changes in force, possibly by coordinating the activity of multiple motors. Alterations in transport affected motion in both apical and basal directions, indicating tight coupling between motors of opposite polarity. Mutations in klar also affect nuclear migration during eye development, suggesting multiple roles for klar-based transport.</p>},
  year = {1998},
  journal = {Cell},
  volume = {92},
  pages = {547-57},
  month = {02/1998},
  language = {eng},
}