@article{2067, keywords = {Gene Expression Regulation, Fungal, Saccharomyces cerevisiae, Trans-Activators, Transcription Factors, Oligonucleotide Array Sequence Analysis, Binding Sites, DNA-Binding Proteins, Promoter Regions, Genetic, Saccharomyces cerevisiae Proteins, DNA Methylation, Calcineurin, Calcium, Response Elements, Sodium}, author = {Hiroyuki Yoshimoto and Kirstie Saltsman and Audrey Gasch and Hong Li and Nobuo Ogawa and David Botstein and Patrick Brown and Martha Cyert}, title = {Genome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae.}, abstract = {
In Saccharomyces cerevisiae, the Ca(2+)/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca(2+) and Na(+), and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a comprehensive analysis of calcineurin/Crz1p-dependent gene expression following addition of Ca(2+) (200 mm) or Na(+) (0.8 m) to yeast. 163 genes exhibited increased expression that was reduced 50\% or more by calcineurin inhibition. These calcineurin-dependent genes function in signaling pathways, ion/small molecule transport, cell wall maintenance, and vesicular transport, and include many open reading frames of previously unknown function. Three distinct gene classes were defined as follows: 28 genes displayed calcineurin-dependent induction in response to Ca(2+) and Na(+), 125 showed calcineurin-dependent expression following Ca(2+) but not Na(+) addition, and 10 were regulated by calcineurin in response to Na(+) but not Ca(2+). Analysis of crz1Delta cells established Crz1p as the major effector of calcineurin-regulated gene expression in yeast. We identified the Crz1p-binding site as 5{\textquoteright}-GNGGC(G/T)CA-3{\textquoteright} by in vitro site selection. A similar sequence, 5{\textquoteright}-GAGGCTG-3{\textquoteright}, was identified as a common sequence motif in the upstream regions of calcineurin/ Crz1p-dependent genes. This finding is consistent with direct regulation of these genes by Crz1p.
}, year = {2002}, journal = {J Biol Chem}, volume = {277}, pages = {31079-88}, month = {08/2002}, language = {eng}, }