@article{1957, keywords = {Escherichia coli, Nitrogen, Time Factors, Kinetics}, author = {Jie Yuan and William Fowler and Elizabeth Kimball and Wenyun Lu and Joshua Rabinowitz}, title = {Kinetic flux profiling of nitrogen assimilation in Escherichia coli.}, abstract = {

We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.

}, year = {2006}, journal = {Nat Chem Biol}, volume = {2}, pages = {529-30}, month = {10/2006}, language = {eng}, }