@article{1712, keywords = {Animals, Drosophila Proteins, Gene Expression Regulation, Developmental, Embryo, Nonmammalian, Male, Base Sequence, Gene Expression Regulation, Molecular Sequence Data, Enhancer Elements, Genetic, Drosophila melanogaster, Transcription Factors, Mesoderm, Repressor Proteins, Ectoderm, Nervous System, Polymerase Chain Reaction, Mutagenesis, Anopheles, Cloning, Molecular}, author = {Michele Markstein and Robert Zinzen and Peter Markstein and Ka-Ping Yee and Albert Erives and Angela Stathopoulos and Michael Levine}, title = {A regulatory code for neurogenic gene expression in the Drosophila embryo.}, abstract = {

Bioinformatics methods have identified enhancers that mediate restricted expression in the Drosophila embryo. However, only a small fraction of the predicted enhancers actually work when tested in vivo. In the present study, co-regulated neurogenic enhancers that are activated by intermediate levels of the Dorsal regulatory gradient are shown to contain several shared sequence motifs. These motifs permitted the identification of new neurogenic enhancers with high precision: five out of seven predicted enhancers direct restricted expression within ventral regions of the neurogenic ectoderm. Mutations in some of the shared motifs disrupt enhancer function, and evidence is presented that the Twist and Su(H) regulatory proteins are essential for the specification of the ventral neurogenic ectoderm prior to gastrulation. The regulatory model of neurogenic gene expression defined in this study permitted the identification of a neurogenic enhancer in the distant Anopheles genome. We discuss the prospects for deciphering regulatory codes that link primary DNA sequence information with predicted patterns of gene expression.

}, year = {2004}, journal = {Development}, volume = {131}, pages = {2387-94}, month = {05/2004}, language = {eng}, }