@article{1416,
  keywords = {chromatin accessibility, multiomics, pituitary, single nucleus analysis, stem cells, transcriptome},
  author = {Zidong Zhang and Michel Zamojski and Gregory Smith and Thea Willis and Val Yianni and Natalia Mendelev and Hanna Pincas and Nitish Seenarine and Mary Amper and Mital Vasoya and Wan Cheng and Elena Zaslavsky and Venugopalan Nair and Judith Turgeon and Daniel Bernard and Olga Troyanskaya and Cynthia Andoniadou and Stuart Sealfon and Frederique Ruf-Zamojski},
  title = {Single nucleus transcriptome and chromatin accessibility of postmortem human pituitaries reveal diverse stem cell regulatory mechanisms.},
  abstract = {<p>Despite their importance in tissue homeostasis and renewal, human pituitary stem cells (PSCs) are incompletely characterized. We describe a human single nucleus RNA-seq and ATAC-seq resource from pediatric, adult, and aged postmortem pituitaries (snpituitaryatlas.princeton.edu) and characterize cell-type-specific gene expression and chromatin accessibility programs for all major pituitary cell lineages. We identify uncommitted PSCs, committing progenitor cells, and sex differences. Pseudotime trajectory analysis indicates that early-life PSCs are distinct from the other age groups. Linear modeling of same-cell multiome data identifies regulatory domain accessibility sites and transcription factors that are significantly associated with gene expression in PSCs compared with other cell types and within PSCs. We identify distinct deterministic mechanisms that contribute to heterogeneous marker expression within PSCs. These findings characterize human stem cell lineages and reveal diverse mechanisms regulating key PSC genes and cell type identity.</p>
},
  year = {2022},
  journal = {Cell reports},
  volume = {38},
  pages = {110467},
  month = {03/2022},
  issn = {2211-1247},
  doi = {10.1016/j.celrep.2022.110467},
  language = {eng},
}