TitleAnalysis of variance of microarray data.
Publication TypeJournal Article
Year of Publication2006
AuthorsAyroles, JF, Gibson, G
JournalMethods Enzymol
Volume411
Pagination214-33
Date Published2006
KeywordsAnalysis of Variance, Animals, Humans, Oligonucleotide Array Sequence Analysis
Abstract

Analysis of variance (ANOVA) is an approach used to identify differentially expressed genes in complex experimental designs. It is based on testing for the significance of the magnitude of effect of two or more treatments taking into account the variance within and between treatment classes. ANOVA is a highly flexible analytical approach that allows investigators to simultaneously assess the contributions of multiple factors to gene expression variation, including technical (dye, batch) effects and biological (sex, genotype, drug, time) ones, as well as interactions between factors. This chapter provides an overview of the theory of linear mixture modeling and the sequence of steps involved in fitting gene-specific models and discusses essential features of experimental design. Commercial and open-source software for performing ANOVA is widely available.

Alternate JournalMeth. Enzymol.