TitleAccumulation of recessive lethal mutations in Saccharomyces cerevisiae mlh1 mismatch repair mutants is not associated with gross chromosomal rearrangements.
Publication TypeJournal Article
Year of Publication2006
AuthorsHeck, JAkiko, Gresham, D, Botstein, D, Alani, E
JournalGenetics
Volume174
Issue1
Pagination519-23
Date Published2006 Sep
KeywordsAdaptor Proteins, Signal Transducing, Base Pair Mismatch, Cell Survival, Chromosome Aberrations, Frameshift Mutation, Fungal Proteins, Gene Rearrangement, Genes, Lethal, Genes, Recessive, Models, Biological, Mutation, Nucleic Acid Hybridization, Probability, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Abstract

We examined mismatch repair (MMR)-defective diploid strains of budding yeast grown for approximately 160 generations to determine whether decreases in spore viability due to the uncovering of recessive lethal mutations correlated with an increase in gross chromosomal rearrangements (GCRs). No GCRs were detected despite dramatic decreases in spore viability, suggesting that frameshift and/or other unrepaired DNA replication lesions play a greater role than chromosomal instability in decreasing viability in MMR-defective strains.
Alternate JournalGenetics