|Title||Accumulation of recessive lethal mutations in Saccharomyces cerevisiae mlh1 mismatch repair mutants is not associated with gross chromosomal rearrangements.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Heck, JAkiko, Gresham, D, Botstein, D, Alani, E|
|Date Published||2006 Sep|
|Keywords||Adaptor Proteins, Signal Transducing, Base Pair Mismatch, Cell Survival, Chromosome Aberrations, Frameshift Mutation, Fungal Proteins, Gene Rearrangement, Genes, Lethal, Genes, Recessive, Models, Biological, Mutation, Nucleic Acid Hybridization, Probability, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins|
We examined mismatch repair (MMR)-defective diploid strains of budding yeast grown for approximately 160 generations to determine whether decreases in spore viability due to the uncovering of recessive lethal mutations correlated with an increase in gross chromosomal rearrangements (GCRs). No GCRs were detected despite dramatic decreases in spore viability, suggesting that frameshift and/or other unrepaired DNA replication lesions play a greater role than chromosomal instability in decreasing viability in MMR-defective strains.