Associate Professor of Physics and the Lewis-Sigler Institute for Integrative Genomics.

Areas of Research: Experimental biophysics; cell shape and collective motility; animal behavior and neuroscience
Department|Program:
  • Physics and the Lewis-Sigler Institute for Integrative Genomics

shaevitz@princeton.edu
Research Lab
Curriculum Vitae
609-258-8177
244 Carl Icahn Laboratory
609-258-5959
Faculty Assistant:
Dawn Capizzi
dcapizzi@princeton.edu
609-258-1617

Research Focus

Cellular and molecular biophysics; Shape, mechanics and motility

The cells that make up all living organisms come in a dizzying array of sizes and shapes, each with unique structural and mechanical properties. When many cells move they contort their bodies to glide along surfaces or swim through fluid. We are interested in the different physical strategies that mother nature has found to create the cellular and sub-cellular structures that perform these amazing feats.

One of the species we study, Spiroplasma melliferum, is a helix barely more than 100 nm across and a few microns long. How does this cell achieve its beautiful shape? When these cells swim they change the helicity of their entire bodies to produce movement. How does this work? How can a cell organize its contents in such a tightly packed space?

It was recently discovered that many, if not all, bacteria use an internal cytoskelton to define their shape, to guide intracellular organization and to produce movement. Our lab studies physical aspects of the prokaryotic cytoskeleton. What are the structural properties of the individual cytoskeletal filaments and how do they contribute to overall cell mechanics? Is mechanical stress induced by the cytoskeleton used to determine cell shape? Are these filaments used as tracks by molecular motors as they are in eukaryotes by kinesin and myosin? These are just some of the basic questions that our lab is interested in.

For this research, we are developing new instrumentation that combines mechanical perturbation of cells and molecules with visualization of key protein and macromolecular structures. Our toolbox includes unique combinations of optical microscopy, fluorescence and deconvolution microscopy, optical trapping, atomic force microscopy, as well as biophysical modeling and simulation.


Selected Publications

  • Balagam R, Litwin DB, Czerwinski F, Sun M, Kaplan HB, Shaevitz JW, Igoshin OA. (2014) Myxococcus xanthus gliding motors are elastically coupled to the substrate as predicted by the focal adhesion model of gliding motility. PLoS Comput Biol. 10: e1003619. Pubmed
  • Deng Y, Sun M, Lin PH, Ma J, Shaevitz JW. (2014) Spatial Covariance Reconstructive (SCORE) super-resolution fluorescence microscopy. PLoS One. 9: e94807. Pubmed
  • Shaevitz JW. (2013) Combining modeling and experiment to understand bacterial growth. Biophys J. 104: 2573. Pubmed
  • Pilizota T, Shaevitz JW. (2013) Plasmolysis and cell shape depend on solute outer-membrane permeability during hyperosmotic shock in E. coli. Biophys J. 104: 2733-42. Pubmed
  • Deng Y, Coen P, Sun M, Shaevitz JW. (2013) Efficient multiple object tracking using mutually repulsive active membranes. PLoS One. 8: e65769. Pubmed
  • Borenstein DB, Meir Y, Shaevitz JW, Wingreen NS. (2013) Non-local interaction via diffusible resource prevents coexistence of cooperators and cheaters in a lattice model. PLoS One. 8: e63304. Pubmed
  • Wang S, Shaevitz JW. (2013) The mechanics of shape in prokaryotes. Front Biosci (Schol Ed). 5: 564-74. Pubmed
  • Pilizota T, Shaevitz JW. (2012) Fast, multiphase volume adaptation to hyperosmotic shock by Escherichia coli. PLoS One. 7: e35205. PubMed
  • van Teeffelen S, Shaevitz JW, Gitai Z. (2012) Image analysis in fluorescence microscopy: bacterial dynamics as a case study. Bioessays. 34: 427-36. PubMed
  • Wang S, Furchtgott L, Huang KC, Shaevitz JW. (2012) Helical insertion of peptidoglycan produces chiral ordering of the bacterial cell wall. Proc Natl Acad Sci. 109: E595-604. PubMed
  • Sun M, Wartel M, Cascales E, Shaevitz JW, Mignot T. (2011) Motor-driven intracellular transport powers bacterial gliding motility. Proc Natl Acad Sci. 108: 7559-64. PubMed
  • Shaevitz JW, Gitai Z. (2010) The structure and function of bacterial actin homologs. Cold Spring Harb Perspect Biol. 2: a000364. PubMed
  • Wang S, Arellano-Santoyo H, Combs PA, Shaevitz JW. (2010) Actin-like cytoskeleton filaments contribute to cell mechanics in bacteria. Proc Natl Acad Sci. 107: 9182-85. PubMed
  • Wang S, Arellano-Santoyo H, Combs PA, Shaevitz JW. (2010) Measuring the bending stiffness of bacterial cells using an optical trap. J Vis Exp. pii: 2012. PubMed
  • Deng Y, Shaevitz JW. (2009) Effect of aberration on height calibration in three-dimensional localization-based microscopy and particle tracking. Appl Opt. 48: 1886-90. PubMed

View complete list of Publications.